The 91 kb conjugative plasmid belonging to IncI1 group carries bla CTX-M-15 and bla TEM-1 genes. coli sequence type 191) exhibited a circular chromosome of 4.73 Mb and two plasmids (91 kb and 4 kb). Four identical chromosomal copies of bla CTX-M-14 are located outside these regions, flanked by IS Ec9 transposases. MDR-1 carries bla TEM-1, tmrB, aac(3)-IId, aadA5, mph(A), mrx, sul1, qacEΔ1 and dfrA17 while MDR-2 harbors aph(3″)-Ib, aph(6)-Id, bla TEM-1, catA1, tet(D) and sul2. The majority of ARGs cluster in close proximity to each other on the chromosome within two separate multidrug-resistance determining regions (MDRs), each flanked by IS 26 transposases. coli sequence type 38) was assembled into a circular chromosome of 5.19 Mb and five plasmids (between 98 kb and 5 kb). The complete genome sequence of strain 631 ( E. This study applied a combination of long-read Oxford Nanopore MinION and short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences of two CTX-M-producing multidrug-resistant Escherichia coli strains isolated from blue mussels ( Mytilus edulis) in Norway, in order to understand the potential for mobility of the detected antibiotic resistance genes (ARGs). This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site.īsrGI is typically used at 37☌, but is even more active at 60☌.Environmental surveillance of antibiotic resistance can contribute towards better understanding and management of human and environmental health. Prolonged incubation with BseRI may lead to degradation of the DNA.īsiWI is typically used at 55☌, but is 50% active at 37☌.
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